T. H. (Henry) Hung [email protected], University of Oxford
📬 For any intended reader only
| 5’ —> 3’ | Len (nt) | Stock solution | Working solution | Storage | |
|---|---|---|---|---|---|
| TSO | GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATrGrGrG | 45 | 75 μM in aliquots | 75 μM | –20°C |
| SMART RT Primer | AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTV | 56 | 100 μM | 10 μM (1:10 dilution) | –20°C |
| SMART cDNA PCR Primer | AAGCAGTGGTATCAACGCAGAGT | 23 | 100 μM | 10 μM (1:10 dilution) | –20°C |
For each reaction:
<aside> ⌛ Aliquot the amount of AMPure XP beads needed for each experiment (after vortexing to resuspend) and let it sit in room temperature for 30 min.
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<aside> ⚠️ A multichannel pipette must be used when dealing with 96-well plates to make sure addition or removal of reagents are rapid enough to maintain consistency across the samples.
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